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KMID : 0848120160410030155
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International Journal of Oral Biology
2016 Volume.41 No. 3 p.155 ~ p.161
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Effect of hypoxia on angiogenesis-related proteins in human dental pulp cells
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Kim Mi-Kyoung
Kim So-Jeong
Kim Yeon
Park Hyun-Joo
Jo Min-Jee
Bae Soo-Kyung
Kim Hyung-Joon
Bae Moon-Kyoung
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Abstract
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Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study, the relative expression of angiogenesis-related proteins was determined in human dental pulp cells using a human angiogenesis proteome profiler array. The proteome profiler array detected differentially expressed angiogenesis-related factors under conditions of hypoxia, which enhances the angiogenic potential of dental pulp cells. We confirmed that hypoxia regulates the mRNA expression of angiogenesis-related factors, including CXCL16 in dental pulp cells. Furthermore, conditioned media of hypoxic pulp cells induced tube-like structures of vascular endothelial cells, which were reduced by the neutralization of CXCL16 function. In conclusion, our data show that angiogenesisrelated factors are differentially expressed by hypoxia in dental pulp cells and suggest that CXCL16 may involve in the revascularization of hypoxic dental pulp.
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KEYWORD
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Human dental pulp cells , angiogenesis , angiogenic factors , hypoxia , CXCL16
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